Congratulations to Maki Inada for her continued successes!Congratulations to Maki Inada for her continued successes!
Sahi C, Lee T, Inada M, Pleiss JA, Craig EA. 2010. Cwc23, an essential J protein critical for pre-mRNA splicing with a dispensable J domain. Mol Cell Biol. Jan;30(1):33-42. View the abstract here.
Inada M and Pleiss JA. 2010. Genome-Wide Approaches to Monitor Pre-mRNA Splicing. Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis. Edited by: Jonathan Weissman; Christine Guthrie and Gerald R. Fink. Methods in Enzymology. Volume 470, Pages 1-892.
Abstract:
Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information—from microarray design to experimental setup to data analysis—to facilitate the widespread use of this technology.
Maki Inada presented "Genome-Wide Analyses of prp8 Alleles Implicated in the Two-State Model for Spliceosome Activitiy" at the RNA Society Meeting in Seattle, WA. June. 2010. Co-authored with Jeffrey Pleiss.
Abstract:
Removal of noncoding introns from pre-rnRNA is catalyzed by the spliceosome, a large multi-component complex I that must be assembled anew for every splicing reaction. Splicing chemistry consists of two separate and sequential transesterification reactions: in the first step the branch site adenosine attacks the 5' splice site to produce the 5' exon and branched lariat intermediate; in the second step the 5' exon attacks the 3' splice site to produce the lariat intron and 1 spliced product. A two-state model for the spliceosome has been previously proposed, in which the conformations of the active site required for the first and second steps are in competition with each other (Query and Konarska 2004). Factors that modulate / and stabilize the first step result in inhibition of the second step and vice versa. A number of such opposiogprp8 alleles that affect the transition between the first and second step have been isolated and characterized to support this model (Query and Konarska 2004, Liu et al. 2007). To further investigate the role that prp8 plays in splicing activation, we have chosen to take two genome-wide approaches in Saccharomyces cerevisiae. First, we have conducted splicing-sensitive microarray analyses to determine the genes that are affected by each of these prp8 alleles. Second, we have taken a high through-put reverse genetic approach known as Synthetic Genetic h a y analysis to identify those factors that are involved in modulating the activity of these prp8 alleles. By determining both the transcripts affected and the complement of factors that genetically interact with each of these prp8 alleles, we will be better able to define how prp8 functions in the splicing pathway. Lastly, strategies for developing a research course for undergraduates utilizing these methods will be discussed.
Read the incredible personal story on our very own, Maki Inada!
"The Power of Fitness: Real Women Share Their Stories." Fitness. 2010.
https://www.ithaca.edu/intercom/article.php/20100706092349628